pUPRT (Toxoplasma)

This plasmid allows targeted replacement of the UPRT locus in Toxoplasma gondii with a construct of choice.

  • AmpR
  • High copy ori
  • Cloning site: 5'-(AscI, XmaI/SmaI, PacI)-3'
  • HA-tag included (in-frame with XmaI/SmaI)
  • GRA2 3'UTR directly follows PacI site
  • Download map in pDRAW format

See the ROP5 virulence paper for its first published use.

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