Isoproponal precipitation of DNA

  1. Add 1% volume of 3M Sodium acetate, pH 5.5.
  2. Add 0.7 volumes isopropanol.
  3. Mix, and spin precipitated DNA in microfuge at room temperature at max speed for 10m.
  4. Carefully remove supernatant.
  5. Add 500 μl 70% ethanol to tube.
  6. Spin 5min in microfuge at max speed.
  7. Carefully remove supernatant with vacuum.
  8. Let tubes dry (open) for a few minutes.
  9. Resuspend in your buffer of choice.
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