Cytomix

Stock Final For 0.5 L For 2 L
1M KCL 120 mM 60 ml 240 ml
1M K PO4 (pH 7.6) 10 mM 5 ml 20 ml
1M HEPES (pH 7.6) 25 mM 12.5 ml 50 ml
100 mM EGTA (pH 7.6) 2 mM 10 ml 40 ml
100 mM CaCl2 0.15 mM 750 µl 3 ml
1M MgCl2 5 mM 2.5 ml 10 ml

pH to 7.6 with KOH, add ddH2O to final volume, filter sterilize

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